Well, I have had quite an eventful week. After little success in viewing any protein we decided to try and reproduce our successful experiment in large quantities and use a new method to view the protein. This will involve solubilising the membranes so that it is easier to apply to the SDS gel, and probably easier for the proteins to transfer to the nitrocellulose paper. However, while we were preparing for this the sequencing for the plasmid we were planning to use came back and it wasn't quite in line, so that was put on hold.
Then yesterday my supervisor decided that seeing as this membrane protein overexpression was proving to be a slow process, he would assign me to a different membrane protein involved in drug resistance while the Masters student continued with the previous one. So, I have been left to plan similar experiments to the last lot, hopefully with more positive results!
Robyn
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